Fig. 4
From: Chronic intermittent hypoxia disrupts protective microgliosis in ischemic proliferative retinopathy
CIH dampens the reactive microglial expansion in ischemic retinopathy. (A) Left: Representative gating of CD45medCD11b+ microglial cells (MC). Right: Quantification of single alive CD45medCD11b+ retinal microglia (n = 11–24 retinas per group; two-way ANOVA and Šídák’s multiple comparisons test * p = 0.0006 PN16 OIR-Norm versus OIR-CIH, $ p = 0.0039 PN18 OIR-Norm versus OIR-CIH). (B) Representative images of central inner retinal microglia, labeled with IBA1 (red) in retinal flatmounts at PN18 and PN30. (C) Left: Representative images of subretinal mononuclear phagocytes (MPs), labeled with IBA1 (red) on RPE/choroidal flatmounts at PN30. Right: quantification of subretinal IBA1 + MPs at PN30 (n = 11–14 eyes per group; one-way ANOVA followed by Šídák’s multiple comparisons test, * p < 0.0001 Normoxia vs. OIR-Norm, $ p < 0.0001 Normoxia vs. OIR-CIH and OIR-Norm vs. OIR-CIH). Scales bars = 100 μm; all values displayed as mean ± SEM, individual points indicate values per retina