Fig. 3
From: Chronic intermittent hypoxia disrupts protective microgliosis in ischemic proliferative retinopathy
OIR induces neurodegeneration and CIH exacerbates functional deficits in retinal signaling. (A) Schematic of the time course of OIR, normoxia, and CIH conditions. (B) Representative B scans of Norm, OIR-Norm, and OIR-CIH retinas at PN24. B scans are from the central retina, near the optic nerve head. Horizontal scale bar = 200 μm, vertical scale bar = 150 μm. (C) Quantification of the thickness of the inner retina, ONL, and IS/OS layers, measured from the OCT B scans (n = 12–24 eyes per group; one-way ANOVA followed by Šídák’s multiple comparisons test, inner retina * p < 0.0001 PN24 and PN30 Normoxia vs. OIR-Norm and Normoxia vs. OIR-CIH; ONL * p = 0.0034 PN24 Normoxia vs. OIR-CIH; IS/OS * p < 0.0001 PN24 Normoxia vs. OIR-Norm, Normoxia vs. OIR-CIH, and OIR-Norm vs. OIR-CIH, * p < 0.0001 P30 Normoxia vs. OIR-Norm and Normoxia vs. OIR-CIH, $ p = 0.0007 P30 OIR-Norm vs. OIR-CIH). (D) Representative ERG waveforms of normoxia (grey), OIR-norm (black), and OIR-CIH (red) at PN30 in response to 4 ms flashes of 3 cd*s/m2. (E) Quantification of a- (left) and b-wave (right) amplitudes at PN30 in response to 4 ms flashes of 3 cd*s/m2 (n = 20–24 eyes per group; one-way ANOVA followed by Šídák’s multiple comparisons test, normalized a-wave amplitude * p < 0.0001 Normoxia vs. OIR-Norm, $ p < 0.0001 Normoxia vs. OIR-CIH and p = 0.0205 OIR-Norm vs. OIR-CIH; normalized b-wave amplitude * p < 0.0001 Normoxia vs. OIR-Norm, $ p < 0.0001 Normoxia vs. OIR-CIH and p = 0.0096 OIR-Norm vs. OIR-CIH). (F) Representative ERG waveforms of normoxia (grey), OIR-norm (black), and OIR-CIH (red) at PN60 in response to 4 ms flashes of 3 cd*s/m2. (G) Quantification of a- (left) and b-wave (right) amplitudes at PN60 in response to 4 ms flashes of 3 cd*s/m2 (n = 11–16 eyes per group; one-way ANOVA followed by Šídák’s multiple comparisons test, normalized a-wave amplitude all p values > 0.05, normalized b-wave amplitude * p < 0.0001 Normoxia vs. OIR-Norm, $ p < 0.0001 Normoxia vs. OIR-CIH and p = 0.0008 OIR-Norm vs. OIR-CIH). All values displayed as mean ± SEM, individual points indicate values per eye. IPL = inner plexiform layer, INL = inner nuclear layer, OPL = outer plexiform layer, ONL = outer nuclear layer, RPE = retinal pigment epithelium, IR = inner retina, PR = photoreceptors, IS/OS = inner and outer segments