Fig. 2
From: Prenatal inflammation exacerbates hyperoxia-induced neonatal brain injury
The combination of prenatal inflammation and postnatal hyperoxia leads to a reduced brain volume and deep cortical layer thinning at P11. After prenatal inflammation at E20 pups were exposed to postnatal normoxia (21% O2) or hyperoxia (80% O2) from P3 to P5 and analysed at P11. A, B Hemisphere volume were determined on cresyl-violet stained sections taken from bregma level − 4.1 ± 0.2 to − 0.5 ± 0.2 mm with a distance of 140 µm between sections. C Cortical thickness of layer V and VI was analysed in TBR1 stained tissue sections. White boxes illustrate the deep cortical layer V–VI depicted on the right side. D, E The ratio of the TBR1 positive deep cortical layer related to the whole hemisphere area was quantified at the hippocampal (3.72 ± 0.7 mm, D) and striatal (− 0.6 ± 0.3 mm, E) level. n = 14–16 rats/ group. *p < 0.05, ***p < 0.001, ****p < 0.0001