Fig. 7

Electrophysiological properties of mitral cells are perturbed during EAE. A Schematic presentation of whole-clamp in mitral cells of the main olfactory bulb. Olfactory receptor neuron = ORN; olfactory nerve layer = ONL; glomerular layer = GL; external plexiform layer = EPL; mitral cell layer = MCL; internal plexiform layer = IPL; granule cell layer = GCL; superficial short axon cell = sSA; periglomerular cell = PGC; external tufted cell = ETC; parvalbumin = PV-positive neuron; granule cell = GC. B Whole-cell currents recorded upon voltage-steps from –100 mV to 20 mV (step size 15 mV), were normalized by cell capacitance to compare current density of mitral cells in healthy (n = 39 cells, n = 25 animals) and acute EAE (d13–16 p.i., n = 43 cells, n = 20 animals). Inset shows increased inward current at negative potentials. Statistics were done by Mann–Whitney U-test; *P < 0.05, **P < 0.01, ***P < 0.001. C Difference in pA/pF throughout the I/V curve between healthy and inflamed mitral cells of the same cohort. D Power density spectrum (in pA²/Hz) in mitral cells of healthy (n = 38 cells, n = 30 animals) vs. acute EAE (d13–16 p.i., n = 23 cells, n = 12 animals). Statistical analysis was performed with two-sampled Kolmogorov–Smirnov test. E Power spectral density in pA²/Hz in healthy mitral cells with potassium-based intracellular solution (potassium glutamate = Kglu; n = 10 cells, n = 7 animals) or cesium-based intracellular solution (CsMeSO₃; n = 10 cells, n = 2 animals). Statistical analysis was performed with two-sampled Kolmogorov–Smirnov test. F Representative traces and quantification of halothane-induced current (pA) in mitral cells of healthy (n = 38 cells, n = 30 animals) and EAE (n = 23 cells, n = 12 animals). Statistical analysis was performed by unpaired, Mann–Whitney U-test; *P < 0.05