Fig. 8

Fatty acid metabolism and mitochondrial function-related targets are upregulated in male mouse PLCγ2-P522R microglia. A Dot plot of normalized enrichment scores for enriched and depleted gene sets in enrichment analyses by GSEA for gene (MSigDB Hallmark, FDR < 0.25) and protein (Wikipathways, FDR < 0.05) expression in CD11b+ microglia isolated from 13-month-old PLCγ2-P522R KI and wild-type (WT) mice. B Heatmap of z scored vst-normalized expression of up- and downregulated differentially expressed genes (DEGs) that were core enrichment genes in a GSEA of gene sets for calcium signaling (Calcium), cholesterol homeostasis (CHOL), fatty acid metabolism (FA), the IFN-α response (IFA), the inflammatory response (INFLAM), oxidative phosphorylation (OXPHOS), and Toll-like receptor signaling (Toll). C Heatmap of z scored vs. normalized expression of significantly up- and downregulated differentially expressed proteins (DEPs, expressed as their encoding gene symbols) derived from a proteomics study. RNA WT n = 3 and PLCγ2-P522R KI n = 4; protein WT n = 5 and PLCγ2-P522R KI n = 6