Fig. 7

PLCγ2-P522R female mice exhibit avoidance responses to open places, bright environments, and sudden unpleasant stimuli. A Body weights of the PLCγ2-P522R (P^ki/ki) mice are lower than those of their wild-type (P^wt/wt) littermates on the APP/PS1-negative background (A-, *p = 0.024) but not on the APP/PS1 hemizygous background (A+). B PLCγ2-P522R does not influence the ambulatory distance traveled in the spontaneous activity test. C P^ki/ki mice spent significantly less time in the center of the novel test box than did control P^wt/wt mice, regardless of the APP/PS1 genotype. D In the passive avoidance test, the P^ki/ki mice showed increased avoidance of the illuminated chamber, which was further accentuated by the APP/PS1 hemizygous genotype. E Even after receiving a foot shock, P^ki/ki mice have a shorter latency to enter the dark chamber as compared to P^wt/wt mice. This tendency was further accentuated by the APP/PS1 hemizygous genotype. F No difference in motor skills was observed between the genotypes, as indicated by the time spent on the rotating rod. G P^ki/ki and P^wt/wt mice have similar thresholds for pain (foot shock), but H) P^ki/ki mice have a stronger behavioral response (crab-like walking backward) to electric foot shock. I) PLCγ2-P522R does not influence spatial learning or memory in the Morris water maze. The APP/PS1 hemizygous mice presented strongly impaired learning in terms of escape latency (F_1,64 = 46.8, p < 0.001). A-/P^wt/wt n = 20, A-/P^ki/ki n = 18, A+/P^wt/wt n = 14, and A+/P^ki/ki n = 11. Two-way ANOVA with Šídák's multiple comparisons test. All the data are presented as the means ± SEMs. Each data point represents an individual mouse