Fig. 6

PLCγ2-P522R variant does not increase the plaque-associated APOE or DAM signature in female APP/PS1 mice. A Analysis of total APOE and APOE areas (µm²) within and surrounding (within 0–10- and 10–20-µm distances from the amyloid plaque outline) β-amyloid plaques revealed no differences between the APP/PS1xPLCγ2-P522R (A+/P^ki/ki) and APP/PS1 (A+/P^wt/wt) mice. A+/P^wt/wt n = 5, A+/P^ki/ki n = 6. Colored disks on the x-axis indicate the analyzed area overlapping (middle circle) and surrounding β-amyloid plaques. The scale bar in the representative immunofluorescence images is 50 μm. Unpaired samples t test. All the data are presented as the means ± SEMs. Each data point represents an individual mouse. B Heatmap of z scored vst-normalized expression of homeostatic as well as stage 1 and stage 2 disease-associated microglia (DAM) signature genes in the bulk temporo-occipital cortex and C) CD11b+ microglia isolated from the whole brain tissue of 13-month-old wild-type (A-/P^wt/wt), A+/P^wt/wt and A+/P^ki/ki female mice. Cortex A-/P^wt/wt n = 1, A+/P^wt/wt n = 4, and A+/P^ki/ki n = 4; CD11b+ A-/P^wt/wt n = 7, A+/P^wt/wt n = 4, and A+/P.^ki/ki n = 4