Fig. 8

Transcriptomic changes in cDC subsets influenced by adoptively transferred PLP-CD8. PLP-CD8-DC and OVA-CD8-DC isolated on day 12 post-EAE induction were prepared for single-cell RNA sequencing (Parse Biosciences). Data were analyzed using the Seurat package in R. A Unsupervised clustering was performed, and clusters were annotated using canonical marker expression. B KEGG pathway analysis using upregulated genes in the CD11b⁺ cDC subset of PLP-CD8-DC showed enrichment of pathways concerning PD-1/PD-L1, Th1 & Th2 differentiation and chemokine and cytokine signaling. C Nichenet analysis identified prioritized ligands for potential intercellular communication between CD11b⁺ cDC and other DC subsets and non-DC antigen-presenting cells