Fig. 7

PLP-CD8-conditioned supernatants promote survival, maturation, and regulatory marker expression in CD11b⁺ cDC. CD11b⁺ cDC (45,000) were incubated with 500 µl of complete media or supernatants from the four conditions of the trans-well assay, sups A-D, for 24 h and analyzed using flow cytometry. A Shows the expression of costimulatory and regulatory markers on live CD11b⁺ cDC incubated with media or different supernatants. B Frequency of live CD11b⁺ cDC was significantly higher following incubation with media or supernatants from conditions involving PLP-CD8 (B-D). C Compares the expression of indicated markers plotted as relative expression (RE) normalized to media. Expression of costimulatory and regulatory markers on live CD11b.⁺ cDC was significantly higher following incubation with supernatants from conditions involving PLP-CD8 (B-D) (N = 3, One-Way ANOVA with multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001)