Fig. 3
PLP-CD8 promote cDC subsets to adopt a mature and regulatory phenotype in vitro. Splenic DC from PLP-immunized mice were incubated with increasing ratios of PLP-CD8 or OVA-CD8 (1:1.25 to 1:5, DC: CD8+) in the presence of PLP178-191 (20 µg/ml) and rIL-2 (10 pg/ml) for 24 h. Freshly isolated DC (direct ex vivo) and DC incubated without CD8+ T cells (No CD8+) were used as controls. The figure shows the expression of costimulatory and regulatory markers on live cDC1 A) and CD11b+ cDC B) at DC: CD8, 1: 5. Marker expression was quantified as geometric mean fluorescence intensity (gMFI) and plotted as the relative expression (RE) normalized to the direct ex vivo condition. Interaction with PLP-CD8 enhanced the expression of costimulatory and regulatory markers on both cDC1 and CD11b+ cDC (N = 3, One-Way ANOVA with multiple comparisons, *p < 0.05, **p < 0.01, ***p < 0.001, ***p < 0.0001)