Fig. 2

PLP-CD8-DC exhibit mature and regulatory phenotype post-EAE induction. PLP or OVA-CD8 were transferred one day before EAE-induction in the recipient mice. PLP-CD8-DC and OVA-CD8-DC were isolated on days 1, 3, 6 and 13 post-EAE induction, and stained with Live-Dead Blue and surface markers for the phenotypic characterization of conventional DC (cDC) subsets using flow cytometry. Expressions of costimulatory and regulatory markers on live cDC subsets were quantified with geometric mean fluorescence intensity (gMFI) and plotted as the relative expression (RE) normalized to OVA-CD8-DC. A By day 6 post-EAE induction, we observed PD-L2 upregulation on both cDC1 and CD11b⁺ cDC subsets of PLP-CD8-DC. B By day 13 post-EAE induction, the cDC1 subset of PLP-CD8-DC showed higher expression of PD-L2 and enrichment of CD86⁺ PD-L2⁺ population. C The CD11b⁺ cDC subset of PLP-CD8-DC displayed upregulation of all costimulatory and regulatory markers and higher co-expression of CD86 with CD83 and PD-L2 (N = 3, Two-Way ANOVA with multiple comparisons, T-test, ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001)