Fig. 3

HIV-1-infected microglia induce an increase in the reactive astrocyte population with elevated p-NF-kB levels. A Representative flow cytometry dot plot showing the population of reactive astrocyte (GFAP +) positive for p-NF-KB in M-BOs after 25 days of incubation in the four treatment conditions. B Graph quantifying the percentage of GFAP + cells in M-BOs in the four treatment conditions obtained by flow cytometry analysis. C Immunofluorescent staining for Dapi (blue) and GFAP (red) on slides of M-BOs in all conditions (top panels). Immunofluorescent staining for Dapi (blue) and S100B (green). Uninfected M-BOs vs HIV-1 M-Bos with an amplification from the same condition. D Graph quantifying the percentage of GFAP positive cells expressing p-NF-KB in M-BOs in the four treatment conditions obtained by flow cytometry analysis. Each organoid treated is an independent experiment and is represented by a single point in the graphs across for the four treatment conditions: Uninfected (n = 4), HIV-1 (n = 3), HIV-1 + ART (n = 3), and HIV-1 + ART Interrupted (n = 3). *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001,****p ≤ 0.0001