Fig. 2

Cerebellar neuro-axonal and synaptic structural integrity in cuprizone-fed mice. a NF200-IHC of the Purkinje cell layer (left panel) and quantification (right panel) showing preserved PC numbers in naive and CUP5W. Purkinje cell numbers were normalized to the proximal perimeter of the molecular layer corresponding to the Purkinje cell layer. Each point represents average measurements from a single animal (naive n = 12; CUP5W n = 10). b. Representative images of MAP2-IHC and quantification showing a significant decrease in MAP2⁺-area in the granule cell layer (naive n = 4; CUP5W n = 6). c. Left panel: High magnification confocal images of the molecular layer fluorescently labelled with anti-calbindin (yellow) and anti-vGlut1 (magenta) antibodies. Nuclei are counterstained with DAPI (white). Right Panel: Quantification pre- (above) and postsynaptic area (below) as percentage of cerebellar cortex. Each point represents average values for a single animal (naive n = 7; CUP5W n = 8). d. Representative electron microscopic images of the molecular layer showing individual synapses (yellow asterisks) in naive and CUP5W mice (left panel), upon quantification (right panel) no significant differences in synaptic density could be observed. Each point represents average values for a single animal (naive n = 5; CUP5W n = 5). Whiskers represent mean ± SEM. P values were obtained after Mann Whitney test for a, c and d or two-way ANOVA with Sidak’s multiple comparisons for b. Asterisks represent significant p-values (*p < 0.05, **p < 0.01, ***p < 0.001, **** p < 0.0001)