Fig. 8

Sgp130 maintains splenic immune cell mIL-6Rreduced brain F4/80 macrophage phosphorylated STAT3 expression at day 7 after MCAO in female animals. Increase in MFI mIL-6R on splenic (A) macrophages, (B) Ly6C^hi monocytes, (C) Ly6G neutrophils and (D) CD3 T cells. (E) Representative gating strategy for brain microglia and F4/80 macrophages. (F)CD45^intCD11b⁺ microglia count, (G) F4/80 macrophage count, (H) MFI mIL-6R on F4/80 macrophages, (I) Decrease in MFI pSTAT3 on F4/80 macrophages. Data presented as mean ± SEM. n = 3–6/gp. Two group comparisons were analyzed by Unpaired t-test with Welch’s correction. *p < 0.05, **p < 0.01