Fig. 3

Microglia and EAU-related DE genes in retinal transcriptome of EAU mice. A Venn diagram comparing the expression of the top DE transcripts (Log2-FC gene expression >2 and p value <0.05) from EAU d14 (blue circle) and d21 (red circle) with a microarray dataset from primary activated microglial cells isolated from degenerating Rs1h-/Y retinas (green circle) to identify microglia-specific genes in EAU (Supplemental Table 4). Microglia-associated genes identified in the subset of DE genes are written in italic. B Genes selected for qRT-PCR analysis are grouped into the subclasses: complement, antigen presentation, interferon, inflammation, and immunoregulation. n-fold gene expression is displayed as box-plot (Log10 n-fold gene expression; target gene). Statistical differences were analyzed via * ANOVA (parametric), or.^# Kruskal–Wallis test (nonparametric; p < 0.05)