Fig. 6
Transcriptomic analysis of TBEV infection in the CNS of mice with varying susceptibility. (A) Numbers of upregulated and downregulated differentially expressed genes (DEGs) in infected samples versus controls across all mouse strains analyzed. The significance thresholds were set to Padj < 0.05 and |log2FC| > 1. (B) Venn diagrams of upregulated and downregulated unique and overlapping DEGs between the mouse strains. The 450 upregulated genes shared across strains represent the general immune response elicited upon infection in all strains. (C) Volcano plots showing DEGs in comparisons of infected and control samples across all mouse strains (|log2FC| >1, FDR < 0.05). (D) Top 50 DEGs of each strain between mock-infected control and TBEV-infected samples showing genes clustered in groups based on expression across strains. (E) Visualization of Gene Ontology overrepresentation analysis of DEGs, combining results across all mouse strains, showing unique and shared terms related to viral infection. The results were filtered for Padj < 0.1, and results with count > 3 are displayed in the plot. (F) Heatmap of selected genes categorized based on their function and results from GO enrichment analysis of shared and unique genes. Mean and scaled expression values in replicates are shown. Abbreviations: BP – biological process, CC – cellular component, MF – molecular function