Fig. 1

Generation and characterization of CO-iMs generated from iPSC-Ru. A Schematic flow diagram indicating the timeline as well as all developmental stages for generation of COs and CO-iMs. B Representative bright field images of a CO and a CO-iM through different stages of their development. Scale bars, 100 µm. C Diameter sizes of COs and CO-iMs measured at key time points during their differentiation (n = 5 per time point). D Left, representative multiplex IF image of a CO-iM stained for nucleus (Hoechst, white), microglia (TMEM119, red), neurons (green, Tuj1) and astrocytes (GFAP, blue). Scale bar, 100 µm. Panel on the right showing individual staining for indicated markers and a merged image from all. Scale bars, 500 µm. E, F Zoomed insets of i), and ii) regions in (D). Scale bars, 100 µm