Fig. 9

Revealing the Effects of RACK1-p.L206P Mutation on Phagocytic Function and Inflammatory Response in Microglial Cells. A Immunofluorescence detection of Rack1 and Iba-1 expression in the mouse Hipp, scale bar = 100 μm, with the right image displaying a statistical graph of positive cells. B Confocal microscopy examination of phagocytic capability in microglial cells, with yellow arrows indicating phagocytic cell bodies and red asterisks representing microglial cell nuclei labeled with Hoechst, scale bar = 25 μm, and the right image showing a statistical graph of microglial cells with phagocytic ability. C−D Quantification of MerTK and Rab7 expression in the mouse Hipp using RT-qPCR (C) and Western blot (D). E ELISA measurement of TNF-α, IL-1β, and IL-6 levels in the mouse Hipp. F Immunofluorescence detection of IL-1β and Iba-1 colocalization in the mouse Hipp, scale bar = 15 μm, with the right image displaying a statistical graph of positive cells. G−H Assessment of iNOS and COX2 expression in the mouse Hipp through RT-qPCR (G) and Western blot (H). Each group consisted of 6 mice, and values are expressed as mean ± standard deviation. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001