Fig. 8

Analysis of Seizure Phenotypes in Mice with Different Genotypes. A Sanger sequencing of three different genotypes of mice. B−C Quantification of Rack1 expression in the Hipp of mice with different genotypes using RT-qPCR (B) and Western blot (C), with 6 mice in each group. D Limb clasping during tail suspension test, with the left image showing a negative response and the right image showing a positive response. E Bar graph depicting the number of negative and positive responses in tail suspension test for mice of different genotypes, with 30 mice in each group. F Steps for audiogenic seizure induction (left) and bar graph showing the number of mice with audiogenic seizures for different genotypes, with 30 mice in each group. G SHIRPA abnormality scores for 30 mice in each group. H−I Total seizure counts (H) and total duration of seizures during EEG monitoring (I) for different genotypes: P206/P206: n = 21, L206/P206: n = 15, L206/L206: n = 20. J The single spike at the onset of seizure followed by irregular spike-wave patterns. K Rapid rhythmic activities with fluctuating amplitudes. L Bar graph showing rapid rhythmic waveforms in two mutant mouse groups, P206/P206: n = 20, L206/P206: n = 13. Values are presented as mean ± standard deviation. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001