Fig. 7

Exploring the Impact of Different Genotypes of HMC3 Cells on HPPNCs. A Fluorescence image of GFP-labeled HPPNCs cells, with red arrows indicating neurons that died during the experiment and white arrows indicating neurons that survived until the end of the experiment. The right image shows a statistical graph of GFP-positive cells, scale bar = 25 μm. B Quantification of Cleaved-CASP3 expression by Western blot. C Immunohistochemical detection of Cleaved-CASP3 expression, scale bar = 50 μm, with the right image showing a statistical graph of positive cells. D Detection of apoptosis in HPPNCs cells using flow cytometry, with the right image showing a statistical graph of apoptotic cell numbers. E Immunofluorescence detection of PSD95/VGLUT2 colocalization in HPPNCs cells, scale bar = 5 μm, with the right image showing a statistical graph of PSD95/VGLUT2 positive cells. F−H Whole-cell patch-clamp recordings of sEPSCs (F), analysis and comparison of sEPSC amplitudes (G) and frequencies (H). I Representative AP traces of neurons under different currents, displaying the firing frequency of AP in neurons with injected currents ranging from 0 to 150 pA. All cell experiments were repeated three times, and values are presented as mean ± standard deviation. * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001