Fig. 3
From: APOEε4 alters ApoE and Fabp7 in frontal cortex white matter in prodromal Alzheimer's disease
FC WM ApoE and Fabp7 positive cells colocalize with different glial cell markers in AD carriers and non-carriers. Double-immunofluorescence images showing ApoE reactive cells (red) (A, G), (C, I), (E, K) that colocalize with the astrocytic glial fibrillar acidic protein (GFAP; blue) (B, H), the microglial ionized calcium-binding adaptor molecule 1 (Iba1; green) (C, D), and the oligodendrocyte marker, oligodendrocyte transcription factor 2 (Olig2; green) (F, L). Note that not all Iba1 and Olig2 positive cells express ApoE. Scale bar in A-L = 10 µM. Triple-immunofluorescent showing single Fabp7 (blue) (M–Q), ApoE (red) (N–R), GFAP (green) (O–S) cells and merged images (pink and yellow) (P, T). Insets show higher magnification images of Fabp7, ApoE and GFAP positive cells (white arrows). Note the consistent co-localization of GFAP and ApoE, while Fabp7 colocalizes only with ApoE. Scale bar in panel P, T = 25 μm and inset = 10 μm