Fig.6
From: Targeting NF-kappaB-inducing kinase shapes B-cell homeostasis in myasthenia gravis
Effect of NIK inhibitor B022 on B cell function under MG sorted B cell culture system in vitro. A The peak plots represent flow gating diagram of B cell antigen presenting marker (CD80, CD86, GITR, HLA-DR and CD95). B MFI of CD80, CD86, GITR, HLA-DR and CD95 between medium and B022 group (n = 6). C The contour plots represent flow gating diagram of B cell associated cytokines (IL-6, IL-10, GM-CSF and TNF-α). D The percentage of IL-6, IL-10, GM-CSF and TNF-α between medium and B022 group (n = 6). E The dot plots represent flow gating diagram of plasmablast and plasma cells. F The percentage of plasmablast and plasma cells between medium and B022 group (n = 6). G The peak plots represent flow gating diagram of NFκB2. H MFI of NFκB2 between medium and B022 group (n = 6). I–J Titers of IgM and IgG from cultured supernatant between medium and B022 group (n = 6). (K) Relative expression of plasma cell related transcription factors (IRF4, Blimp-1, XBP-1, ZBTB20, PAX5, BACH2 and IRF8) between medium and B022 group (n = 6). Data are displayed as means ± SD. Statistical analysis was conducted by independent sample t-test (*P < 0.05, **P < 0.01, ***P < 0.001, ns: non-significant)