Fig. 2

Schematic representation of histological analysis of targeted markers. (A) Analyses were performed on transverse spinal cord cross-sections. (B) Spinal cord sections were stained with Eriochrome Cyanine-Neurofilament (EC-NF) or Hematoxylin-Eosin (H&E) to identify areas of frank tissue pathology. (C) Lesioned areas traced in EC-NF (red line) or H&E (yellow line) stained sections were superimposed on adjacent tissue sections for inflammatory cell quantification. (D) Immunohistochemistry for GFAP, IBA-1, CD68, neutrophils, T cells, and B cells. (E) Cellular quantification was performed by applying the respective method of analysis such as algorithm-based thresholding to generate proportional area or manual counting. (F) Data normalization and statistical analysis. All lesion tracing and data analysis was performed on Halo v2.2.1870