Fig. 7

tPA-NMDAR1 interaction is necessary to induce DA-neuron degeneration in the SN. Representative images of the SN staining with (A) TH and (C) MHC-I, and the respective quantification of (B) DA-neuron survival and (D) MHC-I fluorescence intensity fold change in the SN 4 weeks after rAAV2-hα-SYN injection in tPA Ala-BAC mice treated with glunomab or isotype control (6 ug/day) (n = 5). (E) Confocal image of the SN showing CD3⁺ cells (red) in contact with TH⁺ cell bodies and axons. (F) Quantification of CD3⁺ T cells associated with the SNpc 4 weeks after rAAV2-hα-SYN in tPA Ala-BAC mice treated with glunomab or isotype control (n = 5). These experiments were conducted using 14- to 16-week-old tPA Ala-BAC mice. Data are shown as mean ± SEM, N.S = not significant, *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. (B) 2-tailed t-test; (D, F)1-way ANOVA followed by Tukey post hoc test. Scale bar= (A, C) 250 μm, (E) 20 μm