Fig. 6

Difluorosamine improves neurogenesis and oligodendrogenesis over 14 days of ICH. A, B Representative confocal images of day 14 perihematomal areas labelled with SOX2 (green), Ki67 (red), nestin (blue) in control (A) and DIF (B) mice. C–E Bar graphs comparing number of SOX2⁺ cells (C), SOX2⁺ Ki67⁺ (D), the percentage of nestin + cells in lesion ROI (E). F, G Representative confocal images of day 14 perihematomal areas labelled with Olig2 (red), PDGFRα (blue), CC1 (green) in control (F) and DIF (G) mice. The lower left corner inside the dotted lines is the lesion core. Scale bar = 50 μm. H–J Quantifications comparing number of Olig2⁺ cells (H), OPCs (I) or mature oligodendrocytes (J) per mm² of lesion ROI between control and DIF mice. Data are presented as the mean ± SEM of 6 mice. Each dot represents mean of 4 locations analyzed per mouse. Unpaired two-tailed Student’s t-test; ns: not significant. Significance indicated as *p < 0.05, **p < 0.01, ***p < 0.001