Table 1 Antibodies used in the study
From: Regulatory T cell expansion prevents retinal degeneration in type 2 diabetes
Primary antibody | Cat. No. | Company | Host | Dilution | |
Brn3a Antibody (C-20) | sc-31,984 | Santa Cruz Biotechnology | Goat | 1:200 | |
Ionized calcium binding adaptor molecule 1 (IBA-1) antibody | ab5076 | Abcam | Goat | 1:200 | |
Recombinant Anti-PKC alpha antibody [133] | ab11723 | Abcam | Mouse | 1:500 | |
Anti-Cone Arrestin Antibody | AB15282 | Sigma | Rabbit | 1:500 | |
Guinea Pig Anti-Synaptophysin | AGP-144 | Alomone | Guinea Pig | 1:150 | |
Anti-Glial Fibrillary Acidic Protein antibody (GFAP) | Z0334 | Agilent | Rabbit | 1:500 | |
Biotinylated Isolectin B4 | VEC.B-1205 | Vector Labs | Biotin | 1:50 | |
Albumin | a90-134a | Bethyl | Goat | 1:100 | |
DAPI (4′,6-diamidino-2-phenylindole) | D3571 | ThermoFisher | 1:1000 | ||
Tyrosine hydroxylase (TH) antibody | AB152 | Merck | Rabbit | 1:100 | |
Choline acetyltransferase (ChAT) | Ab144P | Merck | Goat | 1:100 | |
Recombinant Anti-NLRP3 antibody | ab270449 | Abcam | Rabbit | 1:500 | |
Vinculin antibody | sc-73,614 | Santa Cruz Biotechnology | Mouse | 1:1000 | |
GAPDH antibody | ab8245 | Abcam | Mouse | 1:1000 | |
IL-18 (H-173) antibody | sc-72,954 | Santa Cruz Biotechnology | Rabbit | 1:500 | |
Caspase-1 Antibody and p20 | #2225 | Cell Signaling | Rabbit | 1:1000 | |
RBPMS | PA5-115831 | ThermoFisher | Rabbit | 1:100 | |
CD68 | ab53444 | Abcam | Rat | 1:100 | |
Tmem119 | ab209064 | Abcam | Rabbit | 1:100 | |
Secondary antibody | Company | Cat. No. | |||
Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 | ThermoFisher | A10042 | |||
Donkey anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 647 | ThermoFisher | A-31,573 | |||
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | ThermoFisher | A21202 | |||
Donkey anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 647 | ThermoFisher | A32787 | |||
Donkey anti-Goat IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 568 | ThermoFisher | A11057 | |||
Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129) | Abcam | ab150129 | |||
Donkey Anti-Goat IgG H&L (Alexa Fluor® 647) (ab150131) | Abcam | ab150131 | |||
Goat anti-Guinea Pig Alexa Fluor 568 | Abcam | ab175714 | |||
Goat Anti-Rabbit Immunoglobulins/HRP | Agilent DAKO | P0448 | |||
Streptavidin, Alexa Fluor™ 647 conjugated | ThermoFisher | s21374 | |||