Fig. 3

Animals undergoing Treg expansion exhibited reduced DR-related inflammatory features. A-E Analysis of Iba-1⁺ microglial cells and GFAP⁺ Müller cells determined by immunofluorescence. Representative image of microglial cells in sections (A) (Iba-1⁺, red), in retinal flatmounts (C) (Iba-1 green, CD68 red) and D, gliotic Müller cells (GFAP⁺ fibers, red). Scale bars = 25 μm in sections; 50 μm in flatmount). B, Quantitative analysis of the total number of Iba-1⁺ cells in all layers of the retina. E, number of GFAP⁺ fibers crossing the IPL per mm. n = 4–8. F-I, Western blot analysis and representative blot of NLRP3 (F), mature form of IL-18 (G), Caspase-1 (H) and p20 Caspase-1 cleaved form (I) (n = 4). Data presented as mean ± s.e.m. *P < 0.05; ** P < 0.01; ***P < 0.005; 1-way ANOVA followed by Tukey’s multiple comparisons test