Fig. 3
PGE2 stimulates elevation of proinflammatory cytokine levels. Representative cytokine arrays treated with hMG-conditioned media after 6 h of stimulation with (A) vehicle or (B) 1 μM PGE2. (C) Significantly altered targets averaged from all arrays (n = 4). Multiple ratio paired T tests with Holm-Å Ãdák post-hoc tests were used for 3C and adjusted P values are shown. (D) IL6, (E) CXCL8, and (F) IL1B qRT-PCR gene expression changes in hMG stimulated with vehicle or elevating PGE2 concentrations for 6 h (n = 3–6). (G) IL-6, (H) IL-8, and (I) IL-1β ELISA protein level changes from media of hMG stimulated with vehicle or elevating PGE2 concentrations for 6 h (n = 2–4). Data represent mean ± SD. One-way ANOVAs with Dunnett post-hoc tests were used for 3D-I. Statistically significant differences are represented as *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns (not significant) P > 0.05