Fig. 2
Prostanoid production from hRMEC in conditions simulating systemic diabetes. hRMEC were stimulated with (A) 24.5 mM l-glucose or d-glucose, (B) 250 μM palmitic acid, or (C) 1 ng/mL recombinant IL-1β or relevant controls for 24 h, then media were collected for LC–MS/MS targeting PGD2, PGE2, PGF2α, 6-keto-PGF1α (PGI2 metabolite) and TXB2 (TXA2 metabolite). Data were normalized as pg prostanoid per μg of total protein from cell lysates (n = 2–6). Data represent mean ± SD. Two-way ANOVAs with Å Ãdák post-hoc tests were used. Statistically significant differences are represented as ****P < 0.0001, ns (not significant) P > 0.05