Fig. 6
Early injection of anti-IL-17 prevents microglia activation and the increase in pro-inflammatory factors in cerebellum of hyperammonemic rats. Representative images of immunohistochemistry against Iba1 in white matter (A) and GFAP (B). The area (C), perimeter (D) and roundness (E) of microglia (Iba1 stained cells) in white matter are expressed in μm2, μm and arbitrary units, respectively. The reduced area, perimeter and roundness reflects prevention of morphological activation of microglia by anti-IL-17 injection. GFAP stained area expressed as percentage of total area (F). Protein content of (G) TNFα, (H) IL1β and (I) IL-10 was analyzed by Western blot. One-way ANOVA followed by Fisher’s LSD post-hoc test was performed to compare all groups. Values are the mean ± SEM of 3 rats/group in A–F and 7 rats/group in (G-I). Values significantly different from control group are indicated by asterisk (*p < 0.05, **p < 0.01) and values significantly different from HA group are indicated by a (a = p < 0.05, aa = p < 0.01, aaa = p < 0.001, aaaa = p < 0.0001)