Fig. 1

Experimental design. A Experiment 1: To assess if transient blocking of peripheral IL-17 affords sustained prevention of motor impairment. Rats were injected in the tail vein with anti-IL-17 or vehicle after 2, 3 and 4 days of hyperammonemia. Motor coordination was analyzed after 2 weeks of hyperammonemia. Rats were sacrificed after 5 weeks of hyperammonemia. B Experiment 2. To analyze underlying mechanisms, rats were injected in the tail vein with anti-IL-17 or vehicle after 2 and 3 days of hyperammonemia and sacrificed at 4th days. Some rats were perfused for immunohistochemistry and immunofluorescence analysis and others for Western blot analysis of protein content and membrane expression. C Experiment 3: To analyze the production of superoxide in endothelial cells in cerebellum, rats were injected with anti-IL-17 as in experiment 2, but, in addition, after 3 days of hyperammonemia rats were also injected i.p. with dihydroethidium (DHE). After 18 h of DHE injection rats were perfused for immunofluorescence analysis