Fig. 6

Merged analysis of immune cell subsets from retina and CDLNs in KD mice. a UMAP plot clustering of retina and CDLN immune cells from NDE and KDE groups. b Bar chart showing the percentages of immune cell subsets form retina and CDLN of NDE and KDE groups derived from scRNA-seq data. c Line charts showing the mean expression of Pim1, Cxcr4, Hif1a, Id2 in retina and CDLN immune cells from NDE and KDE groups. d Representative GO terms and pathways enriched in upregulated DEGs in retina/LN comparison group between NDE and KDE groups. e UMAP plot clustering of retina and CDLN T cells from NDE and KDE groups. f-g Pseudotime trajectory analysis of CD4⁺ T cells from retina and CDLN of NDE and KDE groups. Cells are colored by pseudotime (right), celltype (left) as indicated (f). Cells are colored by celltype, splited by tissues and group as indicated (g). h–k The scatter distribution plot showed the expression alteration of Pim1, Cxcr4, Ilb, Id2 in each group during the pseudotime. The color coded for different tissues