Fig. 1

Engrailed-2 overexpression affects dendritogenesis of PCs developing in slice cultures. Slices prepared from 6-day-old mice and cultured for 6 days were immunohistochemically stained for CALB1 (green) and EN2 (red). Note the simple and sparsely ramified dendrites of Purkinje cells of L7En-2 mice (B) as compared to cells derived from FVB/N wildtype mice (A). Staining for EN2 (red) reveals overexpression of this transcription factor in Purkinje cells of L7En-2 derived slices (red arrow). Endogenous EN2 expression in numerous non-Purkinje cells is visible in cultures from both genotypes. Bar is 100 µm for overviews and 25 µm for inserts