Fig. 7

Taprenepag improved USVs and damage to TPH-2⁺ neurons in offspring exposed to poly(I: C). (A) The total number of calls for newborn pups in 3 min in the isolation-induced distress vocalization assay. (n = 12, “n” represents offspring from different dams). Pregnant mice received 20 mg/kg poly(I: C) at E12.5 and taprenepag treatment (0.5 mg/kg). USV was conducted on P3-P11, regardless of gender. (B) and (C) The mean fluorescence intensity of immunofluorescence staining for TPH-2⁺ (B) and 5-HT (C) in the MRN of newborn pups. (n = 5 dams, respectively). (D) Immunofluorescence staining of TPH-2⁺ neurons and 5-HT in newborn MRN pups at P10. Statistics were calculated by one-way ANOVA with Dunnett’s post hoc test for (D) and (E) and two-way ANOVA with Tukey’s post hoc test for (B). Statistical significance was denoted by *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. The graphs show the mean ± SEM