Fig. 6

Changes in GHRH neurons after MAD2L2 KO in SAH mice. (A) The expression of MAD2L2 in GHRH neurons was higher on day 7 postsurgery in SAH mice than in sham-operated mice. Scale bar = 100 μm. (B) Quantification of the ARC in A. n = 3. (C) The expression of MAD2L2 in GHRH neurons was higher when ADAM9 was injected into the ARC than when it was injected into the PBS. Scale bar = 100 μm. (D) Quantification of the ARC in C. n = 3. (E) After MAD2L2 KO, the protein levels of the JNK pathway markers caspase-1 and MAD2L2 were measured on day 7 postsurgery in SAH mice by Western blotting. GAPDH was used as a loading control. (F-I) Quantification of E. n = 3. (J) After MAD2L2 KO, the expression of MAD2L2 in GHRH neurons was decreased on day 7 postsurgery in SAH mice. Scale bar = 100 μm. (K) Quantification of the ARC in J. n = 3. (L) After MAD2L2 KO, the expression of GSDMD in GHRH neurons was decreased on day 7 postsurgery in SAH mice. Scale bar = 100 μm. (M) Quantification of the ARC in L. n = 3. (N)The expression of GH increased in the KO MAD2L2 + SAH group. n = 3. (O-R) Cognitive ability improved in the KO MAD2L2 + SAH group. n = 10. ARC: Arcuate nucleus. 3 V: The third ventricle. * p < 0.05; ** p < 0.01; *** p < 0.001