Fig. 5

ADAM9 induces pyroptosis in GHRH neurons via the JNK pathway. (A) Protein levels of JNK, ERK and p38 pathway markers were measured on day 7 postsurgery in SAH and sham-operated mice by Western blot. GAPDH was used as a loading control. (B-G) Quantification of A. n = 3. (H) The expression of GSDMD in GHRH neurons was increased when ADAM9 was injected into the ARC. Scale bar = 100 μm. (I) Quantification of the ARC in H. n = 3. (J) Schematic representation of SAH surgery after blocking the JNK pathway. (K) After SP600125 treatment, the expression of GSDMD in GHRH neurons decreased at 7 days after SAH surgery. Scale bar = 100 μm. (L) Quantification of the ARC in K. n = 3. (M) After the use of SP600125, the protein levels of JNK and pyroptosis pathway markers were measured on day 7 postsurgery in SAH and sham‐operated mice by Western blotting. GAPDH was used as a loading control. (N-P) Quantification of M. n = 3. ARC: Arcuate nucleus. 3 V: The third ventricle. * p < 0.05; *** p < 0.001