Fig. 4

GH and cognitive changes in SAH mice after elimination of hypothalamic microglia. (A) Schematic representation of SAH surgery involving the elimination of microglia. (B) After the use of PLX3397, the number of microglia and the expression of ADAM9 decreased at 7 days after SAH surgery compared with those in control chow-fed mice. Scale bar = 100 μm. (C) Quantification of the data in B. n = 3. (D) After the use of PLX3397, the expression of GSDMD in GHRH neurons decreased on day 7 after SAH surgery. Scale bar = 100 μm. (E) Quantification of the ARC in D. n = 3. (F) After the use of PLX3397, the expression of caspase-1 in the ARC decreased at 7 days after SAH surgery. GAPDH expression was used to confirm equal protein loading and blotting. (G) Quantification of F. n = 3. (H) After treatment with PLX3397, the expression levels of 1β, IL-6 and IL-8 were decreased in the ARC at 7 days after SAH surgery. n = 3. (I) The expression of GH increased in the PLX3397 group. n = 3. (J-M) Cognitive ability improved in the PLX3397 group. n = 10. ARC: Arcuate nucleus. 3 V: The third ventricle. * p < 0.05; ** p < 0.01; *** p < 0.001