Fig. 7

PBMT decreases the EAE-induced hyperexcitability of lumbar interneurons. A, B Bright field images of acute lumbar slices with patch-clamp recording pipette. Scale bar, 400 µm. Inset: High magnification images of interneurons in the dorsal horn (DH), scale bar, 50 µm, (A) or ventro-medial (VM) premotor region (B). C, D Representative membrane potential responses to depolarizing pulses at rheobase (top) or twice the rheobasic strength (bottom) for DH (C) or VM (D) neurons from EAE (gray) or EAE-PBMT (red) mice. E, F Quantification of the resting membrane potentials (left), rheobase (middle), and maximum firing frequency (right) of DH (E) or VM (F) neurons from EAE (gray) or EAE-PBMT (red) mice. G, H Cell body cross-sectional area (left) and number of action potentials induced by depolarizing current steps (right) for the same DH (G) or VM (H) neurons as in E, F. E–H: n = 16 DH and n = 12 VM neurons from six EAE mice and n = 12 DH and n = 15 VM from seven EAE-PBMT mice. Data presented as mean ± SEM and analyzed by nonparametric two-tailed Mann–Whitney U test (E, F, G left, H left) or slope comparisons test of simple linear regressions (G right, H right); *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant. See also Figs. S7, S8