Fig. 5

Effects of PBMT in the ventral spinal regions on D17. A Representative confocal images of Thy1 (cyan), LysM (green), CD11c (yellow), Iba1 (red), and GFAP (blue) labeling in the spine of EAE (left) and EAE-PBMT (right) mice at D17. For each marker, whole slice expression (left column) is followed by two zoomed-in views focusing on the regions highlighted by white rectangles (middle and right columns). Scale bar, respectively, 300 µm, 100 µm, and 40 µm. B Raw and segmented views of cell subtypes. C–K Average densities in specific spinal regions for EAE (gray) and EAE-PBMT (red) mice. C Thy1⁺ axons and soma in the white matter (WM) and gray matter (GM), respectively. D Dorsal and ventral densities of LysM⁺ cells. E Average LysM⁺ cell distance to the surface. F, G Dorsal and ventral densities of CD11c⁺ (F) or Iba1⁺ (G) cells. H Percentage of CD11c⁺ among the total Iba1⁺ cells. I, J Percentage of the GFAP⁺ surface in the dorsal and ventral regions (I), in the WM or GM (J). K Proportion of Iba1⁺ cells contacting GFAP⁺ processes (C–K) n = 13 slices for three EAE and for three EAE-PBMT mice. Data presented as mean ± SEM and analyzed by nonparametric two-tailed Mann–Whitney U test; *P < 0.05; **P < 0.01; ***P < 0.001; NS, not significant