Fig. 3

Transcriptional signature of diabetic hyalocytes. (A) A Principal Component Analysis (PCA) plot demonstrates the distribution of hyalocyte samples isolated from the vitreous of patients with PDR (red dots) and control samples (green dots). (B) Heatmap of differentially expressed genes (DEG, log2FC > 2, adjusted p value < 0.5, sorted according to the logFC) between hyalocytes isolated from the vitreous of PDR patients and control hyalocytes. Color coding of the transcripts according to the z-score (deviation from a gene’s mean expression in standard deviation units). (C) Readplot of DEG in diabetic hyalocytes (upregulated genes in red, downregulated genes in green, not differentially expressed genes in grey; the most strongly expressed genes from each group are labeled; also labeled are the biliverdin reductases A and B, BLVRA and BLVRB). (D) Top 10 most significantly upregulated Gene Ontology (GO) biological process (BP) clusters on the basis of all DEG in diabetic hyalocytes. Color coding of the dots according to the adjusted p value, size of the dots according to the count of transcripts associated with the respective GO term. (E) Top five most highly expressed transcripts in the three most enriched GO terms from D