Fig. 6

IL-6 deficiency attenuates neuroinflammation in 5×FAD mice. (A-B) Representative images showing IBA1 (A) and GFAP (B) staining in the dentate gyrus (DG) and CA1 regions of hippocampal tissue from 5×FAD and Il6^−/−:5×FAD mice. (C-D) Western blot analysis of IBA1 (C) and GFAP (D) in hippocampal tissue from the same groups. (E-F) Quantification of IBA1 (E) and GFAP (F) levels shown in panels C and D. (G) ELISA analysis of IL-1β in hippocampal tissue from the same groups. Each dot represents an individual mouse. Panels (C-F): n = 6 mice per group (mixed sexes). Panel (G): n = 5 mice per group (mixed sexes). Statistical significance for panels (E-F) was determined using Student’s t-test or Welch’s t-test. For panels (G), one-way ANOVA followed by Tukey’s multiple comparisons test was employed. Data are presented as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Ns: not significant. Scale bars: (A-B) 25 μm