Fig. 5

IL-6 deficiency alleviates Aβ burden in 5×FAD mice. (A-C) Representative images showing antibody-positive staining areas for 6E10 (A), Aβ40 (B), and Aβ42 (C) in hippocampal tissue from 6-month-old 5×FAD and Il6^−/−:5×FAD mice. (D-F) Quantification of antibody-positive staining areas for 6E10 (D), Aβ40 (E), and Aβ42 (F). (G) Representative images showing ThS-positive staining areas in hippocampal tissue from the same groups. (H) Quantification of the ThS-positive staining areas in hippocampal tissue from the same groups. Each dot represents an individual mouse. Panels (D), (E), (F), and (H): n = 5 mice per group (mixed sexes). Statistical analysis was performed using Student’s t-test or Welch’s t-test. Data are presented as mean ± S.E.M. Significance levels: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns indicates not significant. (A-C) Scale bar: 250 μm and 25 μm. (G) Scale bar: 50 μm