Fig. 4

IL-6 deficiency mitigates cognitive impairment in 5×FAD mice. (A-B) Total distance traveled (A) and movement speed (B) in the open field test for WT mice, Il6^−/− mice, Il6^−/−:5×FAD mice, and 5×FAD mice. (C-D) Distance traveled (C) and time spent (D) in the central area during the open field test for the same groups. (E-F) Time spent in the open arms (E) and entries into the open arms (F) in the elevated plus maze test for the same groups. (G-H) Swimming speed (G) and escape latency (H) during the five-day training period in the Morris water maze for the same groups. (I-J) Swimming speed (I) and platform crossings (J) during the probe phase of the Morris water maze for the same groups. Each dot represents an individual mouse: WT and Il6^−/− mice (n = 8, mixed sexes); Il6^−/−:5×FAD and 5×FAD mice (n = 10, mixed sexes). Statistical analysis was performed using one-way ANOVA followed by Scheffe’s or Games-Howell tests. Data are presented as mean ± S.E.M. Significance levels are indicated as follows: *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ^★P < 0.05; ns denotes not significant. In panel (H), significance is indicated as * (WT vs. 5×FAD) and ^★ (5×FAD vs. Il6^−/−:5×FAD), with ns denoting no significant difference between WT and Il6^−/− mice