Fig. 3

IL-6 deficiency reduces activation of the STAT3 and cGAS-STING pathways in the hippocampus of 5×FAD mice. (A) Western blot analysis of IL-6 expression in hippocampal tissue from 6-month-old 5×FAD and Il6^−/−:5×FAD mice. (B) Western blot analysis of p-STAT3 and total STAT3 in the same tissue samples. (C) Quantification of the p-STAT3/STAT3 ratio and total STAT3 levels shown in panel (B). (D) Western blot analysis of cGAS and STING expression in hippocampal tissue from the same groups of mice. (E) Quantification of cGAS and STING levels corresponding to panel (D). (F) qRT-PCR analysis of Isg15, Isg20, Ifit1, Ifit2, and Irf7 mRNA levels relative to Gapdh in 5×FAD and Il6^−/−:5×FAD mice. Each dot represents an individual mouse. Panels (A-E): n = 6 mice per group (mixed sexes). Panel (F): n = 5 mice per group (mixed sexes). Statistical significance was determined using Student’s t-test or Welch’s t-test. Data are presented as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Ns: not significant