Fig. 2

Activation of the cGAS-STING pathway in the hippocampus of 5×FAD mice. Western blot analysis of cGAS and STING expression in hippocampal tissue from WT and 5×FAD mice at 4 months (A), 6 months (C), 9 months (E), and 12 months (G) of age. (B), (D), (F), (H) Quantification of cGAS and STING levels shown in panels (A), (C), (E), and (G). Western blot analysis of cGAS and STING at different ages in hippocampal tissue from 5×FAD (I) and WT (K) mice. (J), (L) Quantification of cGAS and STING levels corresponding to panels (I) and (K). (M) qRT-PCR analysis of Isg15, Isg20, Ifit1, Ifit2, and Irf7 mRNA levels relative to Gapdh in 6-month-old WT and 5×FAD mice. Each dot represents an individual mouse. Panels (A-H) and (M): n = 5 mice per group (mixed sexes). Panels (I-L): n = 3 mice per group (mixed sexes). Statistical significance for panels (B), (D), (F), (H), and (M) was determined using Student’s t-test or Welch’s t-test. For panels (J) and (L), one-way ANOVA followed by Tukey’s multiple comparisons test or the Games-Howell test was employed. Data are presented as mean ± S.E.M. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, Ns: not significant