Fig. 6

RS504393 prevents postnatal ethanol-induced stress hyper-response and anxiety-like behavior in adult male and female rats. Time-dependent changes in plasma corticosterone levels following restrain in AD, AD + RS504393, PF, PF + RS504393, AF, AF + RS504393 male and female offspring are shown in panel A and B, respectively. Data are shown as mean ± SEM (n = 5–8/group) and were analyzed using two-way analysis of variance with Tukey’s multiple comparison post hoc test. *, p < 0.05; **, p < 0.01; ***, p < 0.001, ****, p < 0.0001. Effect of prenatal ethanol exposure on anxiety-like behaviors was evaluated by the elevated plus maze (EPM) and open field (OF) in male and female offsprings at PND 60. For EPM analysis, time (sec) in closed and open arms (C) while for OF analysis, time (sec) in corner and center (D) was analyzed in male and female rat offspring. Data are shown as mean ± SEM, n = 5–8 in each group. The data were analyzed using one-way ANOVA followed by the Student Newman-Keuls post hoc test. Significant differences are indicated for AD vs. AF and AF vs. AF + RS504393 groups with *, p < 0.05; **, p < 0.01; ***, p < 0.001