Fig. 2

Chemokine profile of exosomes derived from microglia treated with or without 50 mM ethanol. Rat Chemokine 8-Plex assay was used to determine the percentage changes of various chemokines in exosomes released from microglial cells in primary cultures following ethanol treatment for 24 h (A). Data are mean ± SEM values of three independent experiments. ELISA assay was used to validate the ethanol-induced changes in MCP-1 levels in exosomes of primary microglia (B). Data are mean ± SEM values of three independent experiments and were analyzed using t-test. **, p < 0.01. ELISA assay was used to determine the levels of MCP in exosomes of microglia from pooled three MBH tissues obtained from control (AD, PF) or alcohol-fed (AF) neonatal male (M) and female (F) rats (C). Data are mean ± SEM (N = 3) and were analyzed using two-way analysis of variance with Tukey’s multiple comparison post hoc test. *, p < 0.05; **, p < 0.01