Fig. 1

Flow cytometry gating strategy. (A) Lymphocytes and monocytes were identified on the forward-side-scatter. (B) Monocyte subsets were identified as classical (CD14^highCD16^low), intermediate (CD14^highCD16^high), and non-classical (CD14^lowCD16^high). (C) CD4 + T cells and CD8 + T cells were identified among the lymphocytes. (D) Complement regulatory proteins were gated on the leukocyte subgroups with Boolean sequences, in this example CD35 on CD4 + T cells