Fig. 4

Description and statistical analysis of dendritic spine density and morphology of neurons in the somatosensory cortex (A-D), hippocampal CA1 (E–H), and striatum (I-L) of three groups of mice: Cntnap2 WT, KO mice and PLX5622-treated KO mice. A, E, I, Representative images of dendritic spines of the somatosensory cortex (A), hippocampal CA1 (E), and striatum neurons (I). Scale bar: 10 μm; n = 3–5 mice per group. B, F, J, Representative 3D image of dendritic spines reconstructed by Imaris 9.9 for (A), (E) and (I), respectively. C, G, K, Statistical analysis of the total number of dendritic spines contained in 10 μm dendrites. n = 8–11 cells per group; one-way ANOVA. D, H, L, Statistical analysis of the number of mushroom and stubby dendritic spines contained in 10 μm dendrites. n = 8–11 cells per group; two-way ANOVA. ***p < 0.001, **p < 0.01, *p < 0.05, “ns” denotes no significance. Data are expressed as mean ± SEM