Fig. 2

PLX5622 alleviates hyperactivity and abnormal grooming, and improves nesting behavior of the Cntnap2 KO mice. A, B Open field test. Time spent in center and periphery zone (A) and velocity (B) for four groups of Cntnap2 WT and KO mice treated or not treated by PLX5622. Two-way ANOVA for (A) and one-way ANOVA for (B); n = 8–10 per group. C, Time spent in self-grooming by four groups of mice as labeled in (A). One-way ANOVA; n = 7–11 mice per group. D, E Nesting behavior test. Nestlets after test were documented for all tested animals (D). Scoring was performed using grades ranging from 1 (very poor) to 5 (optimal): grade 1 corresponds to an untouched nestlet, while grade 5 means nestlet fully shredded to form a crater-like, delimited nest (E). One-way ANOVA; n = 8 mice per group. ***p < 0.001; **p < 0.01; “ns” denotes no significance. Data are expressed as mean ± SEM